Creating And Using A New Cell Based Simulation Modifier

This tutorial is automatically generated from TestCreatingAndUsingANewCellBasedSimulationModifierTutorial.hpp at revision 409e06cb314b. Note that the code is given in full at the bottom of the page.

An example showing how to create a new cell-based simulation modifier and use it in a simulation

Introduction

In this tutorial, we show how to create a new cell-based simulation modifier and use this in a cell-based simulation. The simulation modifier class hierarchy is used to implement setup, update and finalise methods in cell-based simulations.

1. Including header files

As in previous cell-based Chaste tutorials, we begin by including the necessary header file and archiving headers.

#include <cxxtest/TestSuite.h>
#include "CheckpointArchiveTypes.hpp"
#include "AbstractCellBasedTestSuite.hpp"

The next header defines a base class for cell-based simulation modifiers. Our new modifier class will inherit from this abstract class.

#include "AbstractCellBasedSimulationModifier.hpp"

The remaining header files define classes that will be used in the cell-based simulation test. We have encountered each of these header files in previous cell-based Chaste tutorials.

#include "AbstractForce.hpp"
#include "HoneycombMeshGenerator.hpp"
#include "NodesOnlyMesh.hpp"
#include "CellsGenerator.hpp"
#include "UniformCellCycleModel.hpp"
#include "TransitCellProliferativeType.hpp"
#include "RepulsionForce.hpp"
#include "OffLatticeSimulation.hpp"
#include "SmartPointers.hpp"
//This test is always run sequentially (never in parallel)
#include "FakePetscSetup.hpp"

Defining the cell-based simulation modifier class

As an example, let us consider a simulation modifier that, at each simulation time step, calculates each cell’s height (y coordinate) in a two-dimensional domain and stores it in in the CellData property as “height”. This might be used, for example in cell-based simulations where cell behaviour is dictated through some form of positional information along a tissue axis.

Note that usually this code would be separated out into a separate declaration in a .hpp file and definition in a .cpp file. Also, while the abstract simulation modifier class is templated over dimensions, for simplicity we hardcode this concrete modifier class to work in 2D only.

class CellHeightTrackingModifier : public AbstractCellBasedSimulationModifier<2,2>
{
    friend class boost::serialization::access;
    template<class Archive>
    void serialize(Archive & archive, const unsigned int version)
    {
        archive & boost::serialization::base_object<AbstractCellBasedSimulationModifier<2,2> >(*this);
    }

The first public method is a default constructor, which simply calls the base constructor.

public:

    CellHeightTrackingModifier()
        : AbstractCellBasedSimulationModifier<2,2>()
    {}

The next public method is a destructor, which calls the base destructor.

    ~CellHeightTrackingModifier()
    {}

Next, we override the UpdateAtEndOfTimeStep() method, which specifies what to do to the simulation at the end of each time step. In this class, we simply call the method UpdateCellData() on the cell population; this method is defined later in the class definition.

    void UpdateAtEndOfTimeStep(AbstractCellPopulation<2,2>& rCellPopulation)
    {
        UpdateCellData(rCellPopulation);
    }

The next overridden method, SetupSolve(), specifies what to do to the simulation before the start of the time loop. In this class, we call UpdateCellData() on the cell population, just as in UpdateAtEndOfTimeStep(). This is needed because otherwise CellData will not have been fully initialised when we enter the main time loop of the simulation.

    void SetupSolve(AbstractCellPopulation<2,2>& rCellPopulation, std::string outputDirectory)
    {

        UpdateCellData(rCellPopulation);
    }

Next, we define the UpdateCellData() method itself. This is a helper method that computes the height (y coordinate) of each cell in the population and stores this in the CellData property.

    void UpdateCellData(AbstractCellPopulation<2,2>& rCellPopulation)
    {

We begin by calling Update() on the cell population, which ensures that it is in a coherent state.

        rCellPopulation.Update();

Next, we iterate over the cell population…

        for (AbstractCellPopulation<2>::Iterator cell_iter = rCellPopulation.Begin();
             cell_iter != rCellPopulation.End();
             ++cell_iter)
        {

…find its height…

            double cell_height = rCellPopulation.GetLocationOfCellCentre(*cell_iter)[1];

…and store this in the CellData item “height”.

            cell_iter->GetCellData()->SetItem("height", cell_height);
        }
    }

Finally, we must override the OutputSimulationModifierParameters() method, which outputs to file any parameters that are defined in the class. In this class, there are no such parameters to output, so we simply call the method defined on the direct parent class (in this case, the abstract class).

    void OutputSimulationModifierParameters(out_stream& rParamsFile)
    {
        AbstractCellBasedSimulationModifier<2>::OutputSimulationModifierParameters(rParamsFile);
    }
};

This concludes the definition of the CellHeightTrackingModifier class.

As mentioned in previous cell-based Chaste tutorials, we need to include the next block of code to be able to archive the simulation modifier object in a cell-based simulation, and to obtain a unique identifier for our new class for when writing results to file.

The identifiers for this class are defined together here, since we can only have each #include once in this source file. Normally the first include and export would go in the class’s header file, and the second #include and export would go in in the .cpp file.

#include "SerializationExportWrapper.hpp"
CHASTE_CLASS_EXPORT(CellHeightTrackingModifier)
#include "SerializationExportWrapperForCpp.hpp"
CHASTE_CLASS_EXPORT(CellHeightTrackingModifier)

The Tests

We now define the test class, which inherits from AbstractCellBasedTestSuite.

class TestCreatingAndUsingANewCellBasedSimulationModifierTutorial : public AbstractCellBasedTestSuite
{
public:

Using the modifier in a cell-based simulation

We conclude with a brief test demonstrating how CellHeightTrackingModifier can be used in a cell-based simulation.

    void TestOffLatticeSimulationWithCellHeightTrackingModifier()
    {

In this case, we choose to create a small NodeBasedCellPopulation comprising 25 cells. We choose a cut-off for mechanical interactions between cells of 1.5 units and add a simple ReplusionForce to the simulation. We use a UniformCellCycleModel to implement some random proliferation in the simulation.

        HoneycombMeshGenerator generator(2, 2, 0);
        boost::shared_ptr<TetrahedralMesh<2,2> > p_generating_mesh = generator.GetMesh();
        NodesOnlyMesh<2> mesh;
        mesh.ConstructNodesWithoutMesh(*p_generating_mesh, 1.5);

        std::vector<CellPtr> cells;
        MAKE_PTR(TransitCellProliferativeType, p_transit_type);
        CellsGenerator<UniformCellCycleModel, 2> cells_generator;
        cells_generator.GenerateBasicRandom(cells, mesh.GetNumNodes(), p_transit_type);

        NodeBasedCellPopulation<2> cell_population(mesh, cells);

        OffLatticeSimulation<2> simulator(cell_population);
        simulator.SetOutputDirectory("TestOffLatticeSimulationWithCellHeightTrackingModifier");
        simulator.SetSamplingTimestepMultiple(12);
        simulator.SetEndTime(20.0);

        MAKE_PTR(RepulsionForce<2>, p_force);
        simulator.AddForce(p_force);

Finally, we add a CellHeightTrackingModifier to the simulation.

        MAKE_PTR(CellHeightTrackingModifier, p_modifier);
        simulator.AddSimulationModifier(p_modifier);

To run the simulation, we call Solve().

        simulator.Solve();
    }
};

It is most straightforward to visualize the results of this simulation in Paraview. Load the file $CHASTE_TEST_OUTPUT/TestOffLatticeSimulationWithCellHeightTrackingModifier/results_from_time_0/results.pvd, and add glyphs to represent cells.

Full code

#include <cxxtest/TestSuite.h>
#include "CheckpointArchiveTypes.hpp"
#include "AbstractCellBasedTestSuite.hpp"

#include "AbstractCellBasedSimulationModifier.hpp"
#include "AbstractForce.hpp"
#include "HoneycombMeshGenerator.hpp"
#include "NodesOnlyMesh.hpp"
#include "CellsGenerator.hpp"
#include "UniformCellCycleModel.hpp"
#include "TransitCellProliferativeType.hpp"
#include "RepulsionForce.hpp"
#include "OffLatticeSimulation.hpp"
#include "SmartPointers.hpp"
//This test is always run sequentially (never in parallel)
#include "FakePetscSetup.hpp"

class CellHeightTrackingModifier : public AbstractCellBasedSimulationModifier<2,2>
{
    friend class boost::serialization::access;
    template<class Archive>
    void serialize(Archive & archive, const unsigned int version)
    {
        archive & boost::serialization::base_object<AbstractCellBasedSimulationModifier<2,2> >(*this);
    }

public:

    CellHeightTrackingModifier()
        : AbstractCellBasedSimulationModifier<2,2>()
    {}

    ~CellHeightTrackingModifier()
    {}

    void UpdateAtEndOfTimeStep(AbstractCellPopulation<2,2>& rCellPopulation)
    {
        UpdateCellData(rCellPopulation);
    }

    void SetupSolve(AbstractCellPopulation<2,2>& rCellPopulation, std::string outputDirectory)
    {

        UpdateCellData(rCellPopulation);
    }

    void UpdateCellData(AbstractCellPopulation<2,2>& rCellPopulation)
    {
        rCellPopulation.Update();

        for (AbstractCellPopulation<2>::Iterator cell_iter = rCellPopulation.Begin();
             cell_iter != rCellPopulation.End();
             ++cell_iter)
        {
            double cell_height = rCellPopulation.GetLocationOfCellCentre(*cell_iter)[1];

            cell_iter->GetCellData()->SetItem("height", cell_height);
        }
    }

    void OutputSimulationModifierParameters(out_stream& rParamsFile)
    {
        AbstractCellBasedSimulationModifier<2>::OutputSimulationModifierParameters(rParamsFile);
    }
};

#include "SerializationExportWrapper.hpp"
CHASTE_CLASS_EXPORT(CellHeightTrackingModifier)
#include "SerializationExportWrapperForCpp.hpp"
CHASTE_CLASS_EXPORT(CellHeightTrackingModifier)

class TestCreatingAndUsingANewCellBasedSimulationModifierTutorial : public AbstractCellBasedTestSuite
{
public:

    void TestOffLatticeSimulationWithCellHeightTrackingModifier()
    {
        HoneycombMeshGenerator generator(2, 2, 0);
        boost::shared_ptr<TetrahedralMesh<2,2> > p_generating_mesh = generator.GetMesh();
        NodesOnlyMesh<2> mesh;
        mesh.ConstructNodesWithoutMesh(*p_generating_mesh, 1.5);

        std::vector<CellPtr> cells;
        MAKE_PTR(TransitCellProliferativeType, p_transit_type);
        CellsGenerator<UniformCellCycleModel, 2> cells_generator;
        cells_generator.GenerateBasicRandom(cells, mesh.GetNumNodes(), p_transit_type);

        NodeBasedCellPopulation<2> cell_population(mesh, cells);

        OffLatticeSimulation<2> simulator(cell_population);
        simulator.SetOutputDirectory("TestOffLatticeSimulationWithCellHeightTrackingModifier");
        simulator.SetSamplingTimestepMultiple(12);
        simulator.SetEndTime(20.0);

        MAKE_PTR(RepulsionForce<2>, p_force);
        simulator.AddForce(p_force);

        MAKE_PTR(CellHeightTrackingModifier, p_modifier);
        simulator.AddSimulationModifier(p_modifier);

        simulator.Solve();
    }
};

Command Line Arguments

Creating And Using A New Cell Cycle Model

Creating And Using A New Cell Based Simulation Modifier

An example showing how to create a new cell-based simulation modifier and use it in a simulation#

Introduction#

1. Including header files#

Defining the cell-based simulation modifier class#

The Tests#

Using the modifier in a cell-based simulation#

Full code#